Protein_Domain

Part:BBa_K4895052:Design

Designed by: Isabella Lirtzman   Group: iGEM23_ASU   (2023-10-09)


DeHa2 (Delftia Acidovorans)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for the organism E.coli (K12). The GC level was optimized to be Medium (0.33 to 0.66). Existing cutsites were removed and no new ones were created for the following enzymes: BbsI.



Source

Delftia Acidovorans

References